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. Author manuscript; available in PMC: 2009 May 6.
Published in final edited form as: Mol Cell Endocrinol. 2006 Oct 19;260-262:109–116. doi: 10.1016/j.mce.2006.03.046

Fig. 2.

Fig. 2

LH/hCG receptor mRNA binding activity of LRBP (luteinizing hormone receptor binding protein) during hCG-induced down-regulation: autoradiogram of RNA gel mobility shift analysis (REMSA). Ovaries were collected from day 5 pseudopregnant, saline-injected (Control), and hCG-injected (12h) rats (D-Reg.). Cytosolic S100 fractions were prepared from the control and hCG down-regulated ovaries. RNA binding reactions were carried out in replicates of four by incubating 1 × 105 cpm of 32P-labeled LHR:102–282 RNA with 50 μg of S100 from the saline-treated control (lanes 1–4) or hCG down-regulated (lanes 5–8) ovaries. (Modified from Kash, J.C. and Menon, K.M.J., 1998, Fig. 6 with permission from the Journal of Biological Chemistry)