Analysis of LRBP purification. Rat ovarian cytosolic proteins (S100-20 μg) (lane 2), partially purified LRBP (20 μg) (lane 3), and two gel-extracted LRBP preparations (1 μg/lane), preparation 1 (lane 4) and preparation 2 (lane 7), were separated on 10% SDS-PAGE and stained with Coomassie Brilliant Blue. (Lanes 1, 5 and 6, prestained standard protein markers (kDa) from Invitrogen.). (Modified from Nair, A.K. and Menon, K.M.J., 2004, Fig. 2 with permission from the Journal of Biological Chemistry)