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11-1F4-binding to plate-immobilized phage, VL Len, and synthetic phage peptides. NaSCN titration curves for antibody binding to (a) phage, protein Len, or (b) synthetic phage peptides. (c) 11-1F4 titration curves for binding to synthetic phage peptides. IC50 and EC50 values were determined from the midpoint of each titration curve. Len, (△); f11, (●); b2, (○); and a12, (♦) phage particles or peptide; and unamplified phage display library control (■).
