Table 2.
Requirements for reconstituted Exo1-independent mismatch repair
| Repair system | 3′ G–T heteroduplex repair, % | 5′ G–T heteroduplex repair, % |
|---|---|---|
| Complete | 53 | 51 |
| − MutSα | 7 | 7 |
| − MutLα | 7 | 8 |
| − RPA | 20 | 18 |
| − PCNA | <3 | <3 |
| − RFC | <3 | <3 |
| − DNA pol δ | <3 | <3 |
| − dNTPs | <3 | <3 |
| − MutLα + MutLαD699N | 3 | 8 |
Complete reactions contained 3′ or 5′ G–T heteroduplex DNA, MutSα, MutLα, RFC, PCNA, RPA, DNA polymerase δ, ATP, and the 4 dNTPs (see Materials and Methods), with omissions as indicated. When present, 10 nM MutLαD699N was substituted for 10 nM MutLα.