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GreB cleavage of put-modified and unmodified ECs. Immobilized ECs were roadblocked at the U-rich pause site and washed as described. GreB was added at the indicated concentrations for 3 min, and the products were analyzed by gel electrophoresis and autoradiography. The amount of uncleaved EC was calculated as a fraction of roadblocked EC. The slight offset of the RNA bands in the first two lanes was not reproducible, and both represent G93 RNAs.
