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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Dev Cell. 2008 Oct;15(4):509–520. doi: 10.1016/j.devcel.2008.07.017

Figure 6. Cells originating in the visceral endoderm contribute to the embryonic gut.

Figure 6

(A) Wholemount view of a 14 somite stage (E8.75) Afp::GFPTg/+ embryo.

(B–D) Transverse sections at different rostrocaudal levels (dashed lines) though the embryo in A.

(E) Wholemount view of a 15 somite stage (E8.75) Ttr::CreTg/+ ; R26::LNL::LacZ+/− embryo.

(F–H) Transverse sections at different rostrocaudal levels (indicated by dashed lines) though the embryo in E. Individual visceral endoderm-derived cells with columnar epithelial morphology constitute part of the hindgut (blue arrowheads); lateral extremities of the gut tube devoid of visceral endoderm-derived cells (red arrowheads). Scale bars = 200 µm in A and E; 100 µm in B–D and F–H.

(I) Diagram illustrating the subdivision of gut tube into foregut (fg), midgut (mg), and hindgut (hg) in a 15 somite stage (E8.75) embryo.

(J) Histograms of visceral endoderm cell contribution to the gut in 14–18 somite stage (E8.75) Afp::GFPTg/+ and Ttr::CreTg/+ ; Z/EGTg/+ embryos. N = 8, 7, 7, 9, 11, and 7 in sequential left-to-right order of bars.