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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Cell. 2009 May 1;137(3):459–471. doi: 10.1016/j.cell.2009.02.027

Figure 1. The N-terminus of hBRE1A Interacts with hBRE1B to Form the hBRE1 Complex.

Figure 1

(A) Intracellular interaction of hBRE1A and hBRE1B. 293T cells were transfected with vectors expressing FLAG-hBRE1A and HA-hBRE1B proteins as indicated, derived cell extracts were incubated with M2 agarose, and bound proteins were visualized by immunoblots with indicated antibodies. (B) Coomassie blue staining and immunoblot analyses of purified hBRE1 complex with indicated antibodies. (C) hBRE1A domains responsible for the hBRE1B interaction. Insect cells were infected with baculoviruses expressing untagged-hBRE1B and the indicated FLAG-hBRE1A fragments, and M2 agarose-purified proteins were visualized by Coomassie blue staining and immunoblots with indicated antibodies (left). A schematic diagram of hBRE1A and derived fragments, with predicted coiled-coil and RING finger domains, and a summary of the hBRE1 interactions are shown (right).