Figure 2.

Inhibition of IL-6 and TNF-α impairs the ability of TLR-dependent cytokines to augment T cell alloimmunity. Conditioned media (CM) were harvested from LPS-activated BMDCs and used to perform an MLR. Inhibition of either IL-6 or TNF-α reduced T cell proliferation (A) or T cell IFN-γ (B) production. Similar results were noted for CD4⁺ and CD8⁺ T cells. Isotype control antibodies did not inhibit T cell responses in this assay (data not shown). *P < 0.05.