Figure 4.

T cell intrinsic IL-6 and TNF-α are dispensable for T cell alloimmunity. (A) Polyclonal T cells were purified from C57BL/6 WT, IL-6^−/−, TNF-α^−/−, and IL-6^−/−TNF-α^−/− mice and stimulated by allogeneic APCs in standard media (lacking cytokines). No differences in T cell proliferation were noted between the groups. (B) Polyclonal T cells (1 × 10⁶) were purified from either C57BL/6 WT or IL-6^−/−TNF-α^−/− mice and adoptively transferred via intravenous tail vein to syngeneic RAG^−/−OTI TCR transgenic mice, which received a BALB/c skin allograft. The rate of graft rejection between the groups was similar. Nonadoptively transferred mice did not reject their allografts.