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Human urine RNA decay rates: mRNAs from normal subjects decayed rapidly at room temperature (RT; top). The rate of decay was slower at 4°C (middle), but, by 6 h, 30 to 40% decay was observed for all mRNAs. The rate of decay was similar for all mRNAs so that expression of podocyte mRNA data divided by a reference gene (AQP2) was relatively constant even after 48 h at 4°C (bottom).
