Table 3.

Calculation of the PFV TS rate.

	Column 1	Column 2	Column 3	Column 4	Column 5	Column 6	Column 7
Selection Medium	Neo + Hygro	Neo	Hygro	TS Phenotype	NON-TS Phenotype (Neo)	NON-TS Phenotype (Hygro)	TS rate per 1 kb
Experiment 1	3,200	7,000	21,000	6,400	3,800	17,800	22.9%
Experiment 2	4,300	16,500	22,500	8,600	12,200	18,200	22.1%
Experiment 3	600	4,100	1,400	1,200	3,500	800	21.8%
average							22.3%

KG81 and KG82 (Fig. 2A) were combined and packaged in 293T cells. Recipient HEK 293 cells were transduced with vector preparations and cells were grown on culture plates supplemented with either neomycin, hygromycin, or both. Colonies were quantified. Column1: number of colonies counted on plates with Neomycin and Hygromycin; column 2: number of colonies counted on plates with Neomycin; column 3: number of colonies counted on plates with Hygromycin; column 4: TS phenotypes (including the double sensitive phenotype that does not grow on the double antibiotics plate) calculated as 2× column 1 (e.g. 2 × 3,200 = 6,400); column 5: Non-TS phenotypes (Neo^R, Hygro^S) calculated as column 2 - column 1 (e.g. 7,000 - 3,200 = 3,800); column 6: Non-TS phenotypes (Neo^S, Hygro^R) calculated as column 3 - column 1 (e.g. 21,000 - 3,200 = 17,800); column 7: TS rate within 1 kb fragment calculated as 100×(column 4/[column 4 + column 5 + column 6]) (e.g. 100 × (6400/[6400+3800+1780]) = 22.9%).