Table 2.

Substitutions made during the creation of E2-Red/Green and E2-Orange.

Construct	Residues differing from DsRed-Express2
E2-Red/Greena	Q42H, A44V, A217T, A219G
E2-Orangeb	Q66T, V71Ac, S179Tc

^a GenBank Accession Number FJ498892

^b GenBank Accession Number FJ498891

^c These mutations were identified during random mutagenesis. All other mutations resulted from targeted mutagenesis.