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. 2009 Apr 16;7:29. doi: 10.1186/1477-7827-7-29

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Copyright © 2009 Clift et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A. agrarius epididymal spermatozoa fail to express CD46 protein. Immunostaining for CD46 (green) in the acrosomal region of epididymal sperm from M. m. musculus (A and C) and A. agrarius (B and D) using an affinity-purified rabbit polyclonal antibody against A. sylvaticus CD46 SCR1 peptide (A and B) or a rat polyclonal antiserum against rat CD46 (C and D). Phase contrast micrographs showing the head morphology of M. m. musculus (E) and A. agrarius (F) sperm. Nuclei are counterstained with DAPI (blue). Scale bars represent 10 μm.