Table 2.

Solubility assessment for representative MK2 constructs.

MK2 Construct		Solubility
Backbone	Mutation(s)	18°C	27°C	37°C
41–364	--	Low	Low	Low
41–364	K64A	Low	Low	Low
41–364	Δ(L220-G236)	High	High	High
41–364	T222E	Low	Low	Low
41–364	K330A	Low	Low	Low
41–364	T334E	Low	Low	Low
41–364	K343A, K344A, K364A	Low	Low	Low
47–366	--	High	High	Medium
47–366	K64A	High	High	Medium
47–366	K84A	High	Med	Low
47–366	Δ(L220-G236)	High	High	High
47–366	T222E	High	High	Low
47–366	T222E, T334E	Low	Low	Low
47–366	T334E	Low	Low	Low

Expression tests were conducted at three temperatures for each construct. Low (<10%), medium (10–50%) and high (>50%) grades were assigned to each construct based on the ratio of soluble/insoluble MK2 as assessed by SDS-PAGE. An example gel is shown in Figure 2.