Table 3.

Summary of pseudoactivated MK2 construct expression, enzymatic activity, and crystallization.

Construct	Yield (mg/L)		Enzymatic Activity (cts/nM)		Crystallography
	Crude	Final	Without p38 activation	With p38 activation	Crystals Obtained?	Diffraction?
MK2(41–364, T222E)	3.0	1.0	0.30	0.34	Yes	No
MK2(47–366, T222E)	6.0	4.0	<0.02	<0.02	Yes	Yes
MK2(47–366, T334E)	4.5	2.0	1.5	1.2	Yes	No