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. 2009 Apr 21;9:26. doi: 10.1186/1471-230X-9-26

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Copyright ©2009 Bramey et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of rHu-EPO on the phosphorylation of JAK-2 in rat livers and hepatocytes. Rat livers were perfused with 8.9 U rHU-EPO/ml and cultured hepatocytes incubated with 10 U/ml (both for 15 minutes). Afterwards, cytoplasmic and nuclear extracts were subjected to Western blotting. As a positive control for EPOR signalling whole cell extracts of the rHu-EPO-responsive cell line UT-7 were used that were treated with (or depleted from) rHU-EPO (10 U/ml for 15 minutes). Total JAK-2 and pJAK-2 were determined for extracts from (A) of perfused liver or (B) total cellular extracts from cultured hepatocytes. The blots represent results of 6 experiments with different animals.