Table 1.
Treatment | Normoxia | Hypoxia (3% O2) |
---|---|---|
Control | 0.11 ± 0.03 fg/μl | 0.05 ± 0.02 fg/μl |
EPO 50 U/ml | 0.09 ± 0.03 fg/μl | 0.04 ± 0.01 fg/μl |
EPO 100 U/ml | 0.08 ± 0.03 fg/μl | 0.04 ± 0.02 fg/μl |
NOTE. Quantitative (RT-)PCR from total RNA was performed as described in Methods after a 24 hours incubation of cultured rat hepatocytes with or without high doses of rHu-EPO under normoxia or hypoxia (3% O2). Data are the means ± SD of 4 separate experiments with hepatocytes different animals each.