Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 20;2:17. doi: 10.1186/1756-8722-2-17

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 McGuirk et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Identification of haptoglobin by LC-MS/MS analysis and database searching. Proteins were excised from the corresponding gel spots and subjected to in-gel digestion. The resulting peptides were extracted from the gel and analyzed by LC-MS/MS. MS/MS data were searched against the human database by Spectrum Mill software to obtain protein identification information. Upper panel: the sequence coverage of haptoglobin by LC-MS/MS analysis. Ten tryptic peptides (sequence underlined) were matched to human haptoglobin by database searching. Lower panel: MS/MS spectra of two of the ten peptides: DIAPTLTLYVGK and VVLHPNYSQVDIGLIK. The peptide sequences were established by extensive b and y ions matched to sequence.