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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cancer Biol Ther. 2009 Apr 22;8(7):618–626. doi: 10.4161/cbt.8.7.7923

Figure 3.

Figure 3

Knockdown of endogenous Axl in MIAPaCa-2 inhibits in vitro cell viability and anchorage independent growth. (A) In vitro cell viability of Axl shRNA-expressing MIAPaCa-2 cells was significantly reduced compared to vector-transfected cells (p < 0.0001), as measured using MTS assay. The MTS assays were performed in triplicate, and mean and standard deviations are plotted. (B) Anchorage independent growth of Axl shRNA-expressing MIAPACA-2 cells, as assessed by colony formation in soft agar, was significantly reduced compared to vector-transfected cells (p = 0.0031). Colony assays were performed in triplicate, and the mean and standard deviations of colony counts were calculated for each condition. (C) Representative soft agar assay of Axl shRNA-expressing MIAPACA-2 compared to vector-transfected cells.