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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cancer Biol Ther. 2009 Apr 22;8(7):618–626. doi: 10.4161/cbt.8.7.7923

Figure 4.

Figure 4

Knockdown of endogenous Axl in MIAPaCa-2 cells inhibits in vitro invasion and migration. (A) Modified Boyden chamber assay (with Matrigel plug) was performed to assess in vitro invasion in MIAPaCa-2 cells. At 72 hours, loss of endogenous Axl function was associated with significant reduction in invasive capacity compared to vector-transfected cells (p < 0.0005), when normalized for cell viability. The histogram represents mean and standard deviation of invasion assay performed in triplicate. (B) Modified Boyden chamber assay (without Matrigel plug) was performed to assess in vitro migration in MIAPaCa-2 cells. At 72 hours, loss of endogenous Axl function was associated with significant reduction in migratory capacity compared to vector-transfected cells (p < 0.0001), when normalized for cell viability. The histogram represents mean and standard deviation of migration assay performed in triplicate.