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. 2009 May;127(1):62–72. doi: 10.1111/j.1365-2567.2008.02933.x

Figure 2.

Figure 2

Tumour necrosis factor (TNF)-α contributes to impaired interleukin (IL)-12 secretion by transforming growth factor (TGF)-β-treated antigen-presenting cells (APCs). Culture supernatants were collected from untreated APCs or those treated with TGF-β (5 ng/ml) and/or TNF-α (5 ng/ml) as described in the Materials and methods. These APCs were harvested from either (a) wild-type (WT) or (b) TNF-α-deficient mice. The amount of IL-12 in culture supernatants was measured by enzyme-linked immunosorbent assay (ELISA) (*P < 0·05 compared with untreated control).