Figure 5.

Expression of tumour necrosis factor receptor 2 (TNF-R2) by transforming growth factor (TGF)-β-treated antigen-presenting cells (APCs) supports a regulatory phenotype of effector T cells. Transgenic ovalbumin (OVA)-specific T cells were co-cultured with OVA-pulsed (a) wild-type (WT) or (b) TNF-R2-deficient APCs that were either untreated or TGF-β-treated (see Materials and methods). Activated T cells were washed and transferred to a fresh well and total TGF-β released in culture supernatants in 24 hr was measured using a bioassay (*P < 0·05 compared with T cells co-cultured with untreated APCs).