Figure 1.

Ca²⁺-stimulated adenylyl cyclase activity as a percentage of basal adenylyl cyclase activity in the striatum depends on AC1 and AC8. A, Striatal adenylyl cyclase activity in naive WT mice from Charles River Laboratories and AC1^+/+ littermate controls under baseline conditions and in the presence of 4 μm free calcium. B, Hippocampal cyclase activity under same conditions as in A. C, Striatal adenylyl cyclase activity in AC1^−/−, AC8^−/−, and DKO mice under baseline and calcium⁺ conditions. All values are means ± SEM of three separate experiments. Individual experiments were performed on tissue pooled from three to five animals with samples analyzed in triplicate. Two-way ANOVA, **p < 0.001, ***p < 0.001 (Bonferroni's test).