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. 1999 Mar 2;96(5):2322–2326. doi: 10.1073/pnas.96.5.2322

Figure 1.

Figure 1

Schematic of MAMA. The hamster cell line, UCW-56, is fused to an FAP lymphoblastoid line, and clones are subsequently selected at 39°C. Only clones that retain human chromosome 5 can grow at this temperature. During the expansion process, hybrids retain at least one human chromosome 5 and usually lose the other copy. Clones then are genotyped to determine which chromosome 5 they contain. Proteins from the fusion clones are used for Western blotting with antibodies reactive with the amino or carboxyl ends of the APC protein. The N-terminal antibody (Human/Rodent APC) reacts with human and rodent APC protein and serves as a loading control. The C-terminal antibody (Human APC) reacts against human but not hamster APC and is used to determine whether full-length APC expression occurs.