Figure 3.

Protein analysis of fusion clones. The Western blots in A were derived from a patient who had a truncating mutation of APC detected with the IVSP assay. Clones 1, 2, and 4 retained allele A and expressed full-length APC whereas clones 3 and 5 retained allele “B” and did not express full-length APC. The blots in B–D were derived from patients in whom no truncating proteins could be detected with IVSP assays. The blots in B were derived from patient 4, who had no full-length APC expression from allele “A” (clones 2 and 4), but who expressed full-length protein from allele “B” (clones 1, 3, and 5). The blots in C were from patient 7, who had reduced expression of APC protein from all fusion clones containing allele “A” compared with those containing allele “B.” The blots in D were derived from patient 9, who had comparable amounts of full-length APC protein expressed from clones retaining either allele “A” or “B.” The asterisk (∗) represents a cross-reactive band present in all hamster cells that is detected with the C-terminal antibody.