Figure 2. Macroscopic currents of WT and E/D mutant hSlo1 with β subunits.

All measurements were made in ∼2 μm[Ca²⁺]_i except for those with the β2 subunit, which were in ∼10 μm[Ca²⁺]_i. The voltage protocols were: for hSlo1 +β1: −80 to +200 mV with 10 mV increments and the repolarization potential at −120 mV; for hSlo1 +β2: −100 to +140 mV with 20 mV increments and the repolarization potential at −80 mV; for hSlo1 +β3b: −80 to +200 mV with 10 mV increments and the repolarization potential at −50 mV; and for hSlo1 +β4: −80 to +200 mV with 10 mV increments and the repolarization potential at −80 mV. In this and other figures, hSlo1 splice variant U11058 was coexpressed with the β2/β2ND subunits.