Table 1.
Steady state kinetic parameters and metal content of HXXC mutants of L1.
| Mutant | kcat (s−1) | Km (μM) | Zn(II) Content |
|---|---|---|---|
| H116Ca | < 0.01 | ND | 0.33 ± 0.01 |
| H121Ca | < 0.01 | ND | 0.11 ± 0.01 |
| H116Cb | 0.38 ± 0.01 | 20 ± 1 | 0.85 ± 0.05 |
| H121Cb | 2.3 ± 0.2 | 72 ± 25 | 0.98 ± 0.05 |
| H116Cc | 0.35 ± 0.01 | 18 ± 1 | NA |
| H121Cc | 33 ± 4 | 99 ± 15 | NA |
| As-isolated/wild-type L1c | 41±1 | 4±1 | 1.90 ± 0.01 |
a
as isolated
b
after adding 2 equivalents of Zn(II) and then dialysis
c
reaction with 100 μM Zn(II) in buffer
ND – not determined
NA – not applicable