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. Author manuscript; available in PMC: 2009 Oct 29.
Published in final edited form as: J Am Chem Soc. 2008 Oct 3;130(43):14207–14216. doi: 10.1021/ja8035916

Table 2.

Steady state kinetics of different metal bound analogs of L1

Species kcat (s−1) Km (μM) Metal Content
ZnZn-L1 39 ± 1 5.9 ± 0.5 2.0 ± 0.1 Zn(II) added
1Zn-L1 30 ± 1 5.5 ± 0.7 1.0 ± 0.1 Zn(II)
1Co-L1 11 ± 1 4.3 ± 0.1 0.9 ± 0.1 Co, 0.10 ± 0.01 Zn(II)
1Fe-L1 2.6 ± 1 53 ± 25 0.9 ± 0.1 Fe, 0.20 ± 0.01 Zn(II)
CoCo-L1a 63 ± 3 20 ± 1 1.80 ± 0.20 Co
FeFe-L1a 0 N/A 1.90 ± 0.01 Fe
ZnCo-L1 26 ± 0.3 2.3 ± 0.1 1.0 ± 0.1 Zn(II); 1.0 ± 0.1 Co
ZnFe-L1b 20 ± 2 3.6 ± 1 1.0 ± 0.1 Zn(II); 1.0 ± 0.1 Fe
ZnFe-L1c 24 ± 3 4.0 ± 1 1.2 ± 0.1 Zn(II); 0.9 ± 0.1 Fe
a

data from Hu et al. 26

b

Fe added to Zn-L1 and then dialysis

c

Zn(II) added to Fe-L1 and then dialysis