Table 1. Summary of transcript and sequence analyses of Avr3c candidate genes in various P. sojae strains.
Straina | Avh28 | Avh27 | Avh432 | Avh26 | ||||
mRNAb | Sequencec | mRNA | Sequence | mRNA | Sequence | mRNA | Sequence | |
P6497 (A) | (+) | Single copy | (+) | Multi-copy | (−) | Single copy | (−) | Singly copy |
P7064 (V) | (+) | Single copy, no polymorphisms | (+) | Multi-copy; numerous non-synonymous SNP | (−) | Single copy, no polymorphisms | (−) | Single copy; single non-synonymous SNP |
P7074 (V) | (+) | Single copy, no polymorphisms | (+) | Multi-copy; numerous non-synonymous SNP | (−) | Single copy, no polymorphisms | (−) | Single copy; single non-synonymous SNP |
P. sojae strains compared were avirulent (A) or virulent (V) on Rps3c plants.
(+), mRNA detected; (−), mRNA not-detected. Transcriptional analysis was performed by RT-PCR on mRNA isolated from infected tissues, 12 h and 24 h post inoculation.
Sequence polymorphisms of P. sojae strains P7064 and P7074 are in comparison to reference strain P6497; SNP, single nucleotide polymorphism.