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. 1999 Mar 2;96(5):2333–2338. doi: 10.1073/pnas.96.5.2333

Figure 4.

Figure 4

FGCV retention in liver as a function of HSV1-tk gene expression. Fourteen adult Swiss–Webster mice were injected by tail vein with 0–2.0 × 109 pfu of AdCMV-HSV1-tk virus and additional control virus to maintain the total viral burden to be fixed to 2.0 × 109 pfu. Forty-eight (±1) hours later, animals received a tail vein injection of FGCV. Animals were sacrificed 180 min later. Livers were removed and liver samples were analyzed for (i) fluorine-18 retained in tissue, (ii) HSV1-tk mRNA levels normalized to glyceraldehyde-3-phosphate dehydrogenase, and (iii) HSV1-TK enzyme levels. (A) HSV1-TK enzyme levels vs. HSV1-tk mRNA levels (y = 0.76x + 0.30, r2 = 0.81). (B) FGCV %ID/g liver vs. HSV1-tk mRNA levels (y = 0.71x + 0.44, r2 = 0.81). (C) FGCV %ID/g liver vs. HSV1-TK enzyme activity (y = 0.79x + 0.31, r2 = 0.71). Each point represents data from a different mouse.