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. 2009 Apr 9;106(17):7257–7262. doi: 10.1073/pnas.0810992106

Fig. 2.

Fig. 2.

Cell fate specification in rbr pollen. (A–E) WT pollen grains. (F–J) rbr pollen grains. (A and F) Bi-cellular pollen grains expressing the vegetative cell marker pLAT52-GFP. (B and G) Fluorescence images of tri-cellular pollen grains stained with DAPI (C and H) The same pollen grains as B and G, respectively, expressing the vegetative cell marker pAC26-H2B-mRFP. WT pollen grain with 1 vegetative cell and 2 sperm cells (B) has only the vegetative cell nucleus expressing pAC26-H2B-mRFP (C). rbr pollen with 1 vegetative cells and 4 germ cells (G). Only the vegetative cell nucleus expresses pAC26-H2B-mRFP (H). (D and I) Tri-cellular pollen grains expressing the germ cell marker pDUO1-DUO1-mRFP. (E and J) In vitro pollen germination. WT pollen produces only 1 pollen tube germinated (E), whereas 2 pollen tubes germinated from the same rbr pollen grain (J). (Scale bars, 10 μm.)