Skip to main content
. 2009 Apr 9;106(17):7113–7118. doi: 10.1073/pnas.0902636106

Fig. 4.

Fig. 4.

Knockdown of SHIP1 or expression of miR-155 in the hematopoietic compartment cause similar MPDs in the bone marrow. (A) Bone marrow was extracted from mice expressing human miR-155 (MG-155, n = 3 mice), mouse miR-155 (MGP-155, n = 4 mice), siSHIP1 (MGP-siSHIP1, n = 3 mice), or control vectors (MG, n = 2 mice or MGP, n = 3 mice) 2 months following bone marrow reconstitution. Total bone marrow cells were assayed for expression of CD11b (Mac1), Ter119, or B220 using flow cytometry. Each dot represents an individual mouse. A P-value of 0.05 or less using a Student's t test was considered statistically significant and indicated with an asterisk. (B) Bone marrow from MGP, MGP-155, or MGP-siSHIP1 mice was smeared and Wright-stained. Photomicrographs are shown (1,000× magnification). (Scale bar, 20 μm.) (C) Representative flow cytometry plots from control, MGP-155 and siSHIP1 vector-containing mouse bone marrow analyzing GFP and CD11b expression.