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. 2009 Apr 20;106(17):7095–7100. doi: 10.1073/pnas.0900879106

Fig. 2.

Fig. 2.

The gene madB is similar to Neurospora wc-2. (A) Aberrant cDNA splicing of the wctA gene in the madB mutants. The cDNAs for the wctA gene from the wild-type strain and 3 madB mutants were amplified by PCR and resolved by gel electrophoresis (Upper). Nucleotide sequence of a single transcript cloned from the wild type and madB strains C109, C111, or C112. The G907A mutation is in bold. Coding nucleotides are shown in uppercase, and intron nucleotides are shown in lowercase font. For the mutant transcripts the zinc-finger domain will be deleted by introduction of a premature stop codon (underlined) (Lower). (B) Genetic evidence that the G907A mutation in wctA is linked to reduced phototropism in madB strains. Progeny (15 derived from 15 independent germinated zygospores) from crosses of strains C109 x A56 or C111 x A56 were examined for phototropism, and 50% of the Petri dishes were photographed. The wctA/madB gene was amplified by PCR and cleaved with RsaI to produce 3 (wild type) or 2 (mutant) fragments. The parents madB (phototropism mutant, sex −, 2 fragments) and A56 (phototropism wild type, sex +, 3 fragments) are not shown. The sex locus shows meiotic recombination in the progeny.