Ly49-specific mAb reactivity of NOD, 129S1 and B6 NK and NKT cells. (a) Splenocyte suspensions of the indicated mouse strains were stained with anti-TCRβ, DX5 and the indicated Ly49-specific mAb. The three left and three right columns show Ly49 surface expression of gated NK (DX5+ TCRβ-) and NKT (DX5+ TCRβ+) cells, respectively. The B6 specificities for each of the mAb used are indicated in parentheses. (b) Collagenase-liberated splenocytes were stained for BST2, SiglecH, and Ly49Q. Plasmacytoid DC were analyzed by gating on BST2+SiglecH+ events. The empty and filled histograms indicate pDC binding of isotype control and anti-Ly49Q mAb, respectively.