5X CB | 2μl |
4NTP mix | 1. 0μL |
CAP | 1. 0μL |
1M DTT | 1. 0μL |
SP6 polymerase | 4 units (0. 4μL) |
RNase inhibitor | 10 units (0. 4μL) |
bovine liver tRNA | 2μg (0. 2μL) |
5X CB | 2μl |
4NTP mix | 1. 0μL |
CAP | 1. 0μL |
1M DTT | 1. 0μL |
SP6 polymerase | 4 units (0. 4μL) |
RNase inhibitor | 10 units (0. 4μL) |
bovine liver tRNA | 2μg (0. 2μL) |