| CB 10X | 2. 5μL |
| 5X EMIX containing 35S met/cys | 5. 0μL |
| WGE | 5. 0μL |
| Myristoyl CoA | 2. 0μL |
| RNase inhibitor | 0. 25μL |
| Creatine Kinase | 0. 25μL |
| tRNA | 0. 25μL |
| transcript (from 3.1) | 5. 0μL |
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