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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 12;378(4):821–825. doi: 10.1016/j.bbrc.2008.11.147

Fig. 1.

Fig. 1

Effects of Conditioned Medium: STAT3 activation in human tumor cells occurs through the action of IL-6. A. 293T cells were treated for 10 min with 2 day conditioned medium (CM) from 231, DU145, HN13 and MCF7 cell lines respectively (left panel, lanes 1–5). pSTAT3 assayed via western blot. hIL-6 specific antibody added to the CM (right panel). B. DU145 cells (top panels, left) or 231 cells (top panel, right) were washed and re-supplied with fresh medium (10 min – 3 h). Western blot for pSTAT3 and total STAT3 (upper and lower panels). C. HN13 cells washed as in B but medium plus anti-IL-6 antibody added. D. pSTAT3 or total STAT3 in HN13; cells treated with IL-6 or OSM antibody for 1 h.