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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 12;378(4):821–825. doi: 10.1016/j.bbrc.2008.11.147

Fig. 3.

Fig. 3

Decrease of serpin B3/B4 mRNA after anti-gp-130 treatment of HN13 cells; a Chromatin Immunoprecipitation (ChIP) shows STAT3 binding to the serpin B3 promoter. A. A fraction of the RNA used (Table 1, supplementary data) was subjected to PCR for serpin B3/B4 mRNA. B. QPCR on cDNA from A. C. Diagram of serpin B3 gene showing proposed STAT3 sites (red bars). D. HN13 cells were treated or not with the Jak1 inhibitor P6 (50μM); ChIP carried out with STAT3 antibody or isotope matched control antibody. Input: 10% of starting extract.