Table 1.
Genotype | 1. jSR lumen width (nm) | 2. jSR contact length (nm) | 3. jSR contacts per IMS | 4. jSR extent (arbitrary units) |
---|---|---|---|---|
Trdn+/+ | 21.7 ± 6.3 (179) | 227 ± 142 (108) | 0.42 ± 0.20 (85) | 95 ± 28 |
Trdn−/− | 37.1 ± 15.0* (192) | 139 ± 62* (82) | 0.30 ± 0.22* (89) | 42 ± 13* |
jSR lumen width was significantly larger and more variable in Trdn−/− cells (see also Fig. 1 C and D). jSR extent (where feet are located) was obtained by multiplying the jSR contact length (column 2) by the frequency of dyads (= jSR contacts per intermyofibrillar space (IMS), column 3). jSR extent was reduced by 56% in the Trdn−/− myocardium. Data are mean ± SD. All data are from 3 mice. In parenthesis are the number of dyads (columns 1 and 2) and number of images (column 3). *, P < 0.001, Student's t test.