Fig. 1.

Antifungal IC₅₀ values (dashed lines) and natural whole tissue concentrations (solid bars) of bromophycolides (A) and callophycoic acids/callophycols (B). Natural whole tissue concentrations were determined by LC-MS analysis of extracts from 4 C. serratus collections of the bromophycolide chemotype (A) and 6 collections of the callophycoic acid/callophycol chemotype (B) (Table S1). Error bars denote 1 SD in metabolite concentration. NSA denotes compounds that were not significantly active at the maximum tested concentration of 300 μM (P > 0.05), as determined by 1-way ANOVA with Dunnett's post test comparison of treatments vs. controls. Among compounds within each chemotype, different letters indicate treatments differing significantly in antifungal IC₅₀ values (F test, P ≤ 0.05). Bromophycolide F and callophycoic acids E and F were neither detected in these extracts nor evaluated for antifungal activity.