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. 2009 Apr 20;106(18):7660–7665. doi: 10.1073/pnas.0812219106

Fig. 3.

Fig. 3.

Overexpression of GUN5 causes spontaneous photobleaching. (A) Representative pictures of WT (Col-0) and 3 GUN5 overexpression lines (GUN5-OX1, GUN5-OX2, and GUN5–OX3). (B) Relative expression of each transgenic gene determined by quantitative RT-PCR. Only GUN5-OX2 and GUN5-OX3 overexpressed the GUN5 gene. (C) Trypan blue staining of GUN5-OX3 leaves. (D) Quantification of photobleaching. Seedlings grown in the dark for various periods of time were transferred to white light for 3 days, and the number of green seedlings was counted. The degree of photobleaching was expressed as percentage of green seedlings. A total of 100 seedlings was used for each time point.