Table 1.
Qualitative and quantitative PCR primers and conditions
| Target | Forward primera | Reverse primera | Probea | Conditions | Reference | |
|---|---|---|---|---|---|---|
| Leishmania | KDNA 120 bp | CCTATTTTACACCAACCCCCAGT | GGGTAGGGGCGTTCTGCGAAA | End-point PCR | 1.25 mmol/l MgCl2; 1 min, 94 °C; 40 cycles – 30 s, 94 °C + 30 s, 58 °C + 30 s, 72 °C | Nicolas et al., 2002 |
| DNA polymerase α 90 bp | TGTCGCTTGCAGACCAGATG | GCATCGCAGGTGTGAGCAC | 5′-FAMCAGCAACAACTTCGAGCCTGGCACC-3′-TAMRA | 3 mmol/l MgCl2; 5 min, 95 °C; 50 cycles – 15 s, 95 °C + 1 min, 65 °C | Bretagne et al., 2001 | |
| Lu. longipalpis | MSLIST6001 150 bp | AAAGGGTGCGAAGTTATTGC | GGGTGGGTTGGACATTCTAC | End-point PCR | 2.5 mmol/l MgCl2; 1 min, 95 °C; 6 cycles – 30 s, 95 °C + 30 s, 53 °C + 45 s, 72 °C; 26 cycles – 30 s, 92 °C + 30 s, 53 °C + 55 s, 72 °C; 30 min, 72 °C | Watts et al., 2001 |
| periodicity 80 bp | ATTTCTTTTCCTTAGGACCATCGA | TAGGACATCTTCGGAAAATTGTTG | 5′-AMTCCTCASAGTCTTTGCATCCACGTTGGTT-3′-TAMRA | 3 mmol/l MgCl2; 5 min, 95 °C; 50 cycles – 15 s, 95 °C + 1 min, 65 °C | Bauzer et al., 2002 | |
a
DNA sequences are given in the standard 5′–3′ direction.