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. Author manuscript; available in PMC: 2009 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 13;15(5):477–484. doi: 10.1038/nsmb.1419

Figure 1.

Figure 1

MMS sensitivity of isw2 nhp10 mutants is due to a prolonged S phase. (a) isw2 nhp10 double mutants grow slowly in the presence of MMS. Wild type (W1588-4c), isw2 (YTT1080), nhp10 (YTT2060), isw2 nhp10 (YTT2109) strains were grown to saturation then 10-fold serial dilutions were plated onto YPD plates with or without 0.02% MMS. (b) S phase is prolonged in MMS-treated isw2 nhp10 mutants. The above strains were arrested in G1 and then released into S phase in the presence of 0.02% MMS as diagrammed. Cells were collected at the indicated times after release and DNA content was determined by flow cytometry (black lines). Gray profiles are from asynchronous cells collected prior to G1 arrest.