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. Author manuscript; available in PMC: 2009 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 13;15(5):477–484. doi: 10.1038/nsmb.1419

Figure 4.

Figure 4

Isw2 and Ino80 may directly facilitate DNA replication. (a) Venn diagram showing the lack of overlap of genes deactivated 1.5 fold in isw2 nhp10 (gray) with genes involved in replication, repair, checkpoint (yellow), and MMS resistance (blue). The 93 genes represented are a collection of genes that are deactivated in isw2 nhp10 mutants in comparison to isw2 and nhp10 single mutants under normal or MMS growth conditions. The Venn diagram encompasses data from 6144 genes. (b) Chromatin immunoprecipitation (ChIP) of Pol1, Nhp10, and Isw2 during S phase. YTT3735 and YTT3736 cells (Pol1-3Flag, Nhp10-13Myc, Isw2-Avi) were arrested in G1 and released into S phase in the presence of 200 mM HU. Cells were harvested at indicated time points in S phase and utilized for ChIP analysis. DNA from immunoprecipitated fractions was analyzed by radioactive PCR with primers corresponding to an early origin (ARS607) and a late origin (ARS1502). ARS1502 does not initiate replication under this experimental condition. The mean and standard deviation of the signals at ARS607 relative to ARS1502 from three biological replicates are presented.