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. Author manuscript; available in PMC: 2009 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2008 Apr 13;15(5):477–484. doi: 10.1038/nsmb.1419

Figure 5.

Figure 5

Isw2 and Ino80 promote replication in the absence of MMS. (a) A clb5 deletion causes a severe growth defect in the absence of Isw2 and Nhp10. Wild type (W1588-4c), isw2 (YTT1080), nhp10 (YTT2060), isw2 nhp10 (YTT2109), clb5 (YTT3402),isw2 clb5 (YTT3434) nhp10 clb5 (YTT3437), and isw2 nhp10 clb5(YTT3441) were grown on YPD media at 30°C for 2 days. (b) A mec1 deletion suppresses the extended S phase of isw2 nhp10 mutants in the presence of 0.02% MMS. mec1 (YTT3003) and mec1 isw2 nhp10 (YTT3048) mutants were treated and their DNA content determined as in Figure 1b. WT and isw2 nhp10 FACS profiles are reproduced from Fig.1b for comparison. Both strains contain an sml1 deletion to suppress lethality of a mec1 deletion38.