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. 2008 Mar 26;2:15–23. doi: 10.2174/1874357900802010015

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2008 Bentham Science Publishers Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. (2) — Processing and half-life of the HPV-16 E7 protein. HaCaT and CaSki cells were pulse labeled with [³⁵S]-methionine-cysteine for 15 min and chased for different times (0, 1, 3 and 6 h). Upper panel: Cell extracts were immunoprecipitated with the anti-E7 polyclonal C89 antibody, samples separated by SDS-PAGE gel and bands visualized by auto-radiography. The arrows showed a 17 kDa (E7a) and a 16 kDa (E7b) bands. The 15 kDa band observed in HaCaT cells is a non-specific band as it is not consistently observed in experiment repetitions. Lower panel: Bands were scanned and plotted in a graph to calculate the half-life of the different forms of E7 protein.