Figure 4.

Multiple sequence alignment of selected representatives of the F₄₂₀-0:γ-l-glutamate ligase family and its homologues from microorganisms known to produce coenzyme F₄₂₀. Sequence identities are highlighted in red and similarities are shown as red letters. The corresponding secondary structure elements of CofE-AF are shown on the top (black). Magenta stars indicate residues that coordinate metal ions, and green dots indicate residues interacting with a nucleotide (GDP). The Gene Bank accession numbers correspond to the following proteins: gi|2648269 CofE-AF from A. fulgidus DSM 4304, gi | 19887484 uncharacterized conserved protein Methanopyrus kandleri AV19, gi|15791326 hypothetical protein VNG2586C Halobacterium sp. NRC-1, gi|15679037 hypothetical protein MTH1019 M. thermautotrophicus str. Delta H, gi|2833574 F₄₂₀-0:γ-glutamyl ligase M. jannaschii MJ0768, gi|45358500 F₄₂₀-0:γ-l-glutamate ligase from M. maripaludis S2. The sequence alignment was performed using the program Multalin⁶² and the Figure was generated using ESPript.⁶³