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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Int J Biochem Cell Biol. 2008 Nov 12;41(5):1216–1227. doi: 10.1016/j.biocel.2008.11.001

Figure 1. β subunit expression in MCF-7 and MDA-MB-231 cells.

Figure 1

(A) Typical gel images of PCR products, taken at the end of the PCR, for SCN1B, SCN2B, SCN3B, SCN4B and cytochrome b5-reductase (Cytb5R) from MCF-7, MDA-MB-231 and PC-3M cells. The PC-3M cell line was used as a positive control for SCN3B expression (Diss et al., 2007). (B) Relative mRNA levels of SCN1B, SCN2B, SCN3B and SCN4B, normalised to Cytb5R by the 2-ΔΔCt method, and expressed as a percentage of the SCN1B mRNA level in MCF-7 cells (fixed as 100 %). Each histobar indicates mean + error propagated through the 2-ΔΔCt analysis (n = 3). Significance is shown in Table 1. (C) Western blot with 70 μg of total protein per lane from MCF-7 and MDA-MB-231 cells, using β1ex and an actinin antibody as a control for loading. The same membrane was stripped and re-blotted.