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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Mol Immunol. 2008 Dec 31;46(6):1222–1228. doi: 10.1016/j.molimm.2008.11.012

Figure 3. HDACi effects on Dicer activity and expression.

Figure 3

A. Cytoplasmic extract from the JAR cell line or recombinant Dicer enzyme was assayed with 50nM TSA, a synthetic pre-miR-122a, and buffer at 37°C for 2 hours. Activity was determined by quantitative RT-PCR of mature miR-122a levels relative to the untreated samples and is presented as Δ CT values. B. JAR cells were treated with valproic acid (250μM for 24hrs) and TSA (50nM for 24hrs). Proteins were collected and analyzed by western blotting for Dicer and βactin levels.