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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Mol Immunol. 2008 Dec 31;46(6):1222–1228. doi: 10.1016/j.molimm.2008.11.012

Figure 7. Apoptosis is not responsible for downregulation of Dicer with IFN-α treatment.

Figure 7

JAR cells were treated with 1000U/mL IFN-αfor 72hrs. For a positive control, JAR cells were treated with 250μM H2O2 for 15minutes. A. The presence of apoptotic cells was analyzed by Annexin V surface staining of treated cells. Treated JAR cultures were stained with Annexin V-FITC (see Materials and Methods). After single cell gating, a marker was set based on the untreated cells and the percentage of cells stained with Annexin V was determined. B. Activation of the apoptotic signaling cascade was analyzed by SDS-PAGE and western blotting for Dicer, Caspase-3, Caspase-8, and βactin.